Microbial Mysteries: Linking Microbial Communities and Environmental Drivers

The Gulf of California is a young ocean undergoing changes including active seafloor spreading, early rifting, and large-scale hydrothermal activity. The rare combination of geological dynamics present in the Gulf of California makes it an ideal place to advance our understanding of deep ocean hydrothermal ecosystems. The basin offers a natural laboratory for investigating changes in microbial communities and dynamics over meter-scale temperature gradients in hydrothermal habitats and between hydrothermal vents and cold seeps along a larger south to north gradient in thermal regime and sedimentation.

Dr. Samantha Joye and her multi-national team will compare the biology, geology, chemistry, and microbiology of sites along the southern to the northern Gulf. The northern area is largely unexplored and the geological characteristics, microbiology, and seafloor fauna are unknown, making a portion of the expedition exploratory in nature. We will explore known and new sites in the Guaymas and Pescadero Basins, building on previous work and diving on new sites. The team aims to identify and quantify habitat-specific microbial populations and assess how those microorganisms interact with the geologic structures and megafauna of vents, seeps, and hydrates. The scientists will visit eight areas in the Gulf, seeking to reveal previously unknown areas with shipboard mapping that is followed by collection of sediment, fluid, and microbiology samples collected with ROV SuBastian. In-situ tools including the ABISS autonomous lander and jumbo osmotic fluid samplers will be used to obtain geochemical data under in situ conditions. Together, these data will help us unravel the microbial mysteries of the Gulf of California.

Microbial Magic
The science team aims to identify and quantify habitat-specific microbial populations and assess how those microorganisms interact with the geologic structures and megafauna of vents and seeps. During the expedition, the team will try a few new things to characterize microbial populations. Genetic sequencing will begin on board the Falkor using an Oxford Nanopore MinION sequencing system. The MinION system allows for genetic sampling using a device the size of a thumb drive. With minimal prep, this amazing sequencer will provide a real time assessment of microbial -omics data. In addition to -omics data, the science team will assess rates of microbial activity using sensitive isotopic tracer techniques and shipboard incubations, and carry out comprehensive geochemical characterization of each habitat.

Back on land, state-of-the-art sequencing tools will yield additional data of metagenomes (DNA), transcriptomes (RNA), and metabolomes (organic carbon metabolites) of the different habitats. These “-omic” methods will provide thorough and robust characterization of microbial activities, and may reveal new microorganisms, new metabolisms, and new microbe-macrofaunal interactions.

Beyond Microbes
The scientists will visit eight sites in the Gulf, looking for hydrothermal plumes above active vents. These plumes will be sampled using the CTD with transect mapping used to better characterize the vent signatures. ROV SuBastian will be deployed to collect sediment, fluid, microbial mats, and benthic fauna samples. Finally, using the ABISS Lander, a novel biogeochemical seafloor observatory, developed in part by the Girguis lab at Harvard, will be used for in-situ sampling.

Special ROV-mounted Jumbo Rapid OSMO samplers, designed by Dr. Joye and colleague Laura Lapham at Maryland, will allow researchers to recover fluids from the deep ocean to determine in situ concentrations of dissolved constituents. The team will also use microscopy to visualize collected microbes and animals.

This expedition will not only advance our understanding of seafloor spreading dynamics, but inform responsible use and protection of valuable marine resources from these rare and underexplored regions.

ROV SuBastian uses fluid samplers will to obtain geochemical data under in situ conditions during an expedition to the Gulf of California in 2018.
A community of Oasisia tube worms in on the seafloor of the Gulf of California. Tubeworms are able to survive due to symbiotic relationships with microbes. These Microbes often form the base of the food system in these environments
The ABISS lander working on the ocean floor (image captured by ROV Subastian).
Max Planck Institute For Marine Microbiology
Harvard University Logo
Coastal Carolina University
University of Wisconsin Madison Logo
Universidad Nacional Autonoma de Mexico

Data & Publications

The resulting shipboard sensor data is being archived at Rolling Deck to Repository and is now available.

ADCP data is curated and archived by University of Hawaii.

Data collected by ROV SuBastian including CTD, photography, navigation, and event logging is available at MGDS.

Biogeochemical data resulting from analysis including Acetate and methanol turnover rates, porewater chemistry, sediment geochemistry, hydrography, water column chemistry, sulifdes and nutrients data is all archived in BCO-DMO.

Water column radiotracer methan oxidation rates are now available in BCO-DMO.

Water column geochemical measurements from CTD samples are now available in BCO-DMO.

Geochemical measurements of porewater from sediment push core samples is now available in BCO-DMO.

In the News

The upside-down pool in the ocean

Yahoo News • July 8, 2020

Scientists Find New Hydrothermal Field

Marine Technology News • April 3rd, 2019

Amazing ‘Mirrors’ Spotted Deep in Sea

Weather Channel • April 3rd, 2019

Something Wondrous (#7)

Axios Science • April 4th, 2019

Found: Hot Pink ‘Pagodas’ at the Bottom of the Sea

Atlas Obscura • April 5th, 2019

Mirror-Like Pools Hidden Under The Pacific Ocean

The Science Times • April 6th, 2019

New Mirror-Like Pools Discovered Deep in the Pacific Ocean

Gizmodo Australia  • April 8th, 2019

Scientists Spot Beautiful Optical Illusion at Bottom of the Sea

Smithsonian Magazine • April 8th, 2019

Under The Sea: Ocean researcher explores an underwater world

UGA Today: Great Commitments • April 16th, 2019